High Performance Liquid Chromatographic Technique and Validation for Determination of Favipiravir in Bulk & Tablet Formulation

Dr. Mirza Shaihed Baig; Ms. Pritee R. Atkar

Title:
High Performance Liquid Chromatographic Technique and Validation for Determination of Favipiravir in Bulk & Tablet Formulation

Authors:
Ms. Pritee R. Atkar | Dr. Mirza Shaihed Baig

Cite This Article :

Ms. Pritee R. Atkar | Dr. Mirza Shaihed Baig "High Performance Liquid Chromatographic Technique and Validation for Determination of Favipiravir in Bulk & Tablet Formulation" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-6 | Issue-7, December 2022, pp.719-725, URL: https://www.ijtsrd.com/papers/ijtsrd52424.pdf

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Abstract :
Favipiravir is an antiviral that is active against many RNA viruses, & also used in COVID-19. A simple, economical, accurate, and precise HPLC method with UV detection was developed to quantify Favipiravir. It was resolved on the C18 column using the mobile phase blend of methanol: acetonitrile: ortho phosphoric acid in an isocratic mode, flow rate of 1.0 mL/min with a proportion of 40:30:30%,v/v/v. The detector wavelength was set at 322 nm. Reverse phase HPLC method, using UV detector is developed for the estimation of Favipiravir API. Used Liquid chromatographic system from Shimadzu comprising of Auto sampler, quaternary gradient pump for constant flow and constant pressure delivery and UV-Visible detector connected to software Lab solutions for controlling the instrumentation as well as processing the generated data. A mixture of Methanol: Acetonitrile: Ortho phosphoric acid in the ratio 40:30:30 v/v/v was used in RP-HPLC as diluent and as a mobile phase.

Keywords :
Favipiravir, Valadation, Accuracy, Precision, LOD

Publication Details:

Unique Identification Number : IJTSRD52424

Published In : Volume-6 | Issue-7, December 2022

Page Number(s) : 719-725

Publisher Name : IJTSRD | www.ijtsrd.com | E-ISSN 2456-6470

Copyright © 2019 by author(s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0)

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